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Primer GC / Tm Analyzer

Free browser-based Primer GC / Tm Analyzer. Process sequence data locally without uploading it to a server.

✓ Runs locally in your browser✓ No account required✓ No sequence or structure data upload
Approx. Tm
—
GC
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Length
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Wallace Tm
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Simple screening estimate: Wallace rule for short primers and a basic GC/length formula for longer primers. It is not a nearest-neighbor, salt- and Mg²⁺-corrected thermodynamic calculation.
Об этом инструменте

Primer GC / Tm Analyzer runs locally in the browser and is designed for quick bioinformatics or cheminformatics calculations.

Common uses
primer tm calculatorprimer melting temperature calculatorprimer gc calculatoroligo tm calculator

Как это работает

  1. 1Paste or type the requested input data.
  2. 2Choose any available options and run the tool.
  3. 3Review the result and copy it into your own workflow if needed.

Часто задаваемые вопросы

Does this tool upload my data?

No. The calculation runs locally in your browser.

Is this a replacement for specialist scientific software?

No. It is intended for quick calculations and screening; validate critical results with your established workflow.

Is this a nearest-neighbor Tm calculation?

No. It is intentionally a simple screening estimate and does not model salt, magnesium, mismatches or nearest-neighbor thermodynamics.

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